selector plasmid Search Results


127553 127555 optional control sgrna  (Addgene inc)
91
Addgene inc 127553 127555 optional control sgrna
A. Conversion table for target gene cut frame and CRISPaint frame selector <t>sgRNA.</t> B. Screenshot of output of the Find CRISPRs tool (www.flyrnai.org/crispr3/web). Arrow, column in a search results table showing the target gene sgRNA cut frame. The example sgRNA shown was used to generate His2Av-mNeonGreen knock-in cell line in Bosch et al. 2019. C. Schematic of gene targeting when using the three CRISPaint frame selector sgRNAs (0, 1, 2).
127553 127555 Optional Control Sgrna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/127553 127555 optional control sgrna/product/Addgene inc
Average 91 stars, based on 1 article reviews
127553 127555 optional control sgrna - by Bioz Stars, 2026-05
91/100 stars
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pcfd5 frame selector 0 1 2  (Addgene inc)
93
Addgene inc pcfd5 frame selector 0 1 2
A. Conversion table for target gene cut frame and CRISPaint frame selector <t>sgRNA.</t> B. Screenshot of output of the Find CRISPRs tool (www.flyrnai.org/crispr3/web). Arrow, column in a search results table showing the target gene sgRNA cut frame. The example sgRNA shown was used to generate His2Av-mNeonGreen knock-in cell line in Bosch et al. 2019. C. Schematic of gene targeting when using the three CRISPaint frame selector sgRNAs (0, 1, 2).
Pcfd5 Frame Selector 0 1 2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcfd5 frame selector 0 1 2/product/Addgene inc
Average 93 stars, based on 1 article reviews
pcfd5 frame selector 0 1 2 - by Bioz Stars, 2026-05
93/100 stars
  Buy from Supplier
pcfd3 frame selector 1  (Addgene inc)
93
Addgene inc pcfd3 frame selector 1
A. Conversion table for target gene cut frame and CRISPaint frame selector <t>sgRNA.</t> B. Screenshot of output of the Find CRISPRs tool (www.flyrnai.org/crispr3/web). Arrow, column in a search results table showing the target gene sgRNA cut frame. The example sgRNA shown was used to generate His2Av-mNeonGreen knock-in cell line in Bosch et al. 2019. C. Schematic of gene targeting when using the three CRISPaint frame selector sgRNAs (0, 1, 2).
Pcfd3 Frame Selector 1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcfd3 frame selector 1/product/Addgene inc
Average 93 stars, based on 1 article reviews
pcfd3 frame selector 1 - by Bioz Stars, 2026-05
93/100 stars
  Buy from Supplier
selector plasmid  (Promega)
90
Promega selector plasmid
A. Conversion table for target gene cut frame and CRISPaint frame selector <t>sgRNA.</t> B. Screenshot of output of the Find CRISPRs tool (www.flyrnai.org/crispr3/web). Arrow, column in a search results table showing the target gene sgRNA cut frame. The example sgRNA shown was used to generate His2Av-mNeonGreen knock-in cell line in Bosch et al. 2019. C. Schematic of gene targeting when using the three CRISPaint frame selector sgRNAs (0, 1, 2).
Selector Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/selector plasmid/product/Promega
Average 90 stars, based on 1 article reviews
selector plasmid - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
frame selector plasmid  (Schmid GmbH)
90
Schmid GmbH frame selector plasmid
A. Conversion table for target gene cut frame and CRISPaint frame selector <t>sgRNA.</t> B. Screenshot of output of the Find CRISPRs tool (www.flyrnai.org/crispr3/web). Arrow, column in a search results table showing the target gene sgRNA cut frame. The example sgRNA shown was used to generate His2Av-mNeonGreen knock-in cell line in Bosch et al. 2019. C. Schematic of gene targeting when using the three CRISPaint frame selector sgRNAs (0, 1, 2).
Frame Selector Plasmid, supplied by Schmid GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/frame selector plasmid/product/Schmid GmbH
Average 90 stars, based on 1 article reviews
frame selector plasmid - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier

Image Search Results


A. Conversion table for target gene cut frame and CRISPaint frame selector sgRNA. B. Screenshot of output of the Find CRISPRs tool (www.flyrnai.org/crispr3/web). Arrow, column in a search results table showing the target gene sgRNA cut frame. The example sgRNA shown was used to generate His2Av-mNeonGreen knock-in cell line in Bosch et al. 2019. C. Schematic of gene targeting when using the three CRISPaint frame selector sgRNAs (0, 1, 2).

Journal: Current protocols in molecular biology

Article Title: Use of the CRISPR-Cas9 system in Drosophila cultured cells to introduce fluorescent tags into endogenous genes

doi: 10.1002/cpmb.112

Figure Lengend Snippet: A. Conversion table for target gene cut frame and CRISPaint frame selector sgRNA. B. Screenshot of output of the Find CRISPRs tool (www.flyrnai.org/crispr3/web). Arrow, column in a search results table showing the target gene sgRNA cut frame. The example sgRNA shown was used to generate His2Av-mNeonGreen knock-in cell line in Bosch et al. 2019. C. Schematic of gene targeting when using the three CRISPaint frame selector sgRNAs (0, 1, 2).

Article Snippet: Materials Cas9-expressing S2R+ cells (S2R+-MT::Cas9, DGRC Stock #268) Schneider’s Medium (see Reagents & Solutions ) Effectene transfection reagent (301427, Qiagen) sgRNA plasmid for each target/design (see Basic Protocol 1 or 2 for design and Support Protocol 1 for synthesis) ssDNA donor (see Basic Protocol 1 , Support Protocol 2 ) or CRISPaint donor plasmid (see Basic Protocol 2 ) For Basic Protocol 2 , frame selector plasmid ( pCFD3-frame_selector_ (0,1,or 2) (Addgene #s 127553–127555) Optional control sgRNA for Basic Protocol 2 , pCFD3-Act5c (GP07595, Addgene # 130278) 6-well tissue culture plates (3516, Costar) Plate Drosophila Cas9-expressing S2R+ cells into 6-well dishes at a concentration of 1.8×10 6 cells/ml (2 ml volume each well, 3.6×10 6 cells/well) in Schneider’s Medium.

Techniques: Knock-In

Troubleshooting

Journal: Current protocols in molecular biology

Article Title: Use of the CRISPR-Cas9 system in Drosophila cultured cells to introduce fluorescent tags into endogenous genes

doi: 10.1002/cpmb.112

Figure Lengend Snippet: Troubleshooting

Article Snippet: Materials Cas9-expressing S2R+ cells (S2R+-MT::Cas9, DGRC Stock #268) Schneider’s Medium (see Reagents & Solutions ) Effectene transfection reagent (301427, Qiagen) sgRNA plasmid for each target/design (see Basic Protocol 1 or 2 for design and Support Protocol 1 for synthesis) ssDNA donor (see Basic Protocol 1 , Support Protocol 2 ) or CRISPaint donor plasmid (see Basic Protocol 2 ) For Basic Protocol 2 , frame selector plasmid ( pCFD3-frame_selector_ (0,1,or 2) (Addgene #s 127553–127555) Optional control sgRNA for Basic Protocol 2 , pCFD3-Act5c (GP07595, Addgene # 130278) 6-well tissue culture plates (3516, Costar) Plate Drosophila Cas9-expressing S2R+ cells into 6-well dishes at a concentration of 1.8×10 6 cells/ml (2 ml volume each well, 3.6×10 6 cells/well) in Schneider’s Medium.

Techniques: Knock-In, Immunostaining, Western Blot, Live Cell Imaging, Clone Assay